Table 1. HPLC condition for analysis

Parameter Condition
HPLC Prominance 20A (Shimadzu, Japan)
Mobile phase Water(0.1% phosphoric acid) : acetonitrile
Column Sunfire C18 (4.6 × 250 mm, 5 μm)
Wavelength 230, 330 nm
Injection volume 10 μL
Oven temperature 35°C
Flow rate 1.0 mL/min