Parameter | Condition |
---|---|
HPLC | Prominance 20A (Shimadzu, Japan) |
Mobile phase | Water(0.1% phosphoric acid) : acetonitrile |
Column | Sunfire C18 (4.6 × 250 mm, 5 |
Wavelength | 230, 330 nm |
Injection volume | 10 |
Oven temperature | 35°C |
Flow rate | 1.0 mL/min |