Table 2. Mobile phase condition of HPLC
Time (min)
A% (0.1% phosphoric acid in water)
B% (acetonitrile)
0
95
5
5
95
5
15
90
10
25
87
13
30
85
15
35
83
17
55
80
20
75
70
30
85
40
60
90
10
90
93
10
90
95
95
5
100
95
5